FIDO-PHI
Fido-Φ, or also known as Free-Fall, is a two-dimensional imaging fluorometer. It falls through the water column taking high-resolution images of the physical and planktonic microstructures that exists in our oceans. It's an autonomous, untethered instrument composed of a high sensitivity, low noise, thermo-electric cooled CCD camera (LUMIS) and a green (532 nm) laser. The laser light, spread as a thin sheet, is used to induce the chlorophyll from the microstructures, which lights as fluorescence. The camera is equipped with special filters to image this fluorescence. The imaging takes place 75 cm below the instrument and therefore in a completely undisturbed medium. The fall rate is ~10 cm/s (adjustable) and images are taken at 2-second interval and stored by the camera controller on its internal hard drive. The Field of view (FOV) is 32 by 32 cm with a 312 micron resolution. There is also sensor data that is saved by the sensor controller. Sensor readings include depth, speed, heading, pitch, roll, temperature, and humidity for all 3 housings (camera, laser, and sensor). The sensor controller also controls the laser and the depth at which the weight is released, set to 100 m, allowing the unit to get back to the surface. All data is downloaded via Ethernet once the unit is retrieved and on deck.
The system is broken down into four major components; camera, laser, sensors, and frame. The camera, laser, and sensor components are contained in separate pressure housings. The sensor component consists of a PC with National Instruments interface boards running LabVIEW. The sensor component monitors depth, heading, pitch, roll, and vital statistics of all three housings (temperature and humidity) in addition to controlling an automated weight release system. The camera component is a silightly modified version of the LUMIS system and consists of a PC with Medoptics interface boards and a highly sensitive camera. The laser component consists of a high power diode pumped 532mn laser with a custom lens. The sensor and camera components are connected via ethernet and are both individualy accessable from and external computer.
- Principal Investigators: Jules Jaffe and Peter Franks
- Chief Engineer: F. Simonet
- Mechanical Engineer: Fred Ulhman - Hydrodynamic aspects, mechanical design/fabrication.
- Software Development: Paul Roberts - Data acquisition, instrument control, GUI
David Zawada - Calibration, data analysis/algorithms. - Optical Consultant: Karl Moore
- Lab Assistants: Mathew Diebolt
- Consultant: Wendy Storms
- Graduate Students: Erdem Karakoylu, David Zawada
References
- Franks, PJS, Jaffe JS. 2008. Microscale variability in the distributions of large fluorescent particles observed in situ with a planar laser imaging fluorometer. Journal of Marine Systems. 69:254-270.
- Citekey Franks2002 not found
- Jaffe, JS, Ohman MD, De Robertis A. 1998. OASIS in the sea: measurement of the acoustic reflectivity of zooplankton with concurrent optical imaging. Deep Sea Research Part II: Topical Studies in Oceanography. 45:1239-1253.
- Jaffe, JS. 1990. Computer Modeling and the Design of Optimal Underwater Imaging-Systems. IEEE Journal of Oceanic Engineering. 15:101-111.
- Palowitch, AW, Jaffe JS. 1995. Optical Serial Sectioned Chlorophyll-Alpha Microstructure. Journal of Geophysical Research-Oceans. 100:13267-13278.
- Palowitch, AW, Jaffe JS. 1995. Optical Serial Sectioned Chlorophyll-Alpha Microstructure. Journal of Geophysical Research-Oceans. 100:13267-13278.





